、 PUNG-LING HUANG合授

課程簡介: 一、 內容 本課程涵蓋下列主題，著重於分子生物在植物生理、植物遺傳與育種上之應用，俾學習者具備研讀植物分子生物學論文之能力。 1. 高等植物之基因組大小及信使RNA複雜性 2. 模式植物---阿拉伯芥 3. 以傳統方法選殖植物基因 4. 以跳動因子標誌法選殖植物基因 5. 植物基因的構造 6. 高等植物之基因調控 7. 葉綠體基因及其調控 8. 不親和性之分子分析 9. 種子蛋白及其基因 10. 休眠與發芽之分子生物學 11. 生長激素控制之生長及基因表達 12. 植物逆境之分子生物學 二、教科書 General 1. Alberts B, Bray D, Lewis J, Raff M, Roberts & Watson JD (1994) Molecular Biology of the Cell, 3rd ed. Garland Publishing Inc, New York & London. 2. Dennis DT & Turpin DH (1990) Plant Physiology, Biochemistry and Molecular Biology. Longman Scientific & Technical. 529pp. 3. Grierson D & Covey SN (1998) Plant Molecular Biology, 2nd ed. Chapman and Hall, New York. 233pp. 4. Lewin B (1997) Genes VI. Oxford University Press, Oxford, New York & Tokyo. Lab manual 1. Draper J, Scott R, Armitage P & Walden R (1988) Plant Genetic Transformation and Gene Expression - A Laboratory Manual. Blackwell Scientific Publication, Oxford, London, Edinburgh, Boston, Palo Alto & Melbourne. 2. Glover DM (1985) DNA Cloning - A Practical Approach, Vols I & II. IRL Press, London. 3. Glover DM (1989) DNA Cloning - A Practical Approach, Vol III. IRL Press, London. 4. Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning - A Laboratory Manual, 2nd ed. Vols 1, 2 & 3. Cold Spring Harbor Laboratory Press, New York. 5. Schuler MA & Zielinski RE ( 1989) Methods in Plant Molecular Biology. Academic Press Inc, San Diego. 6. Shaw CH (1988) Plant Molecular Biology - A Practical Approach. IRL Press, Oxford, Washington DC. 7. Weissbach A & Weissbach H (1988) Methods for Plant Molecular Biology. Academic Press Inc, San Diego. 三、 成績評量方式 期終考試、期末考試與學期報告各佔三分之一。 四、 預修課程 植物生理、生物化學。

This course will focus on the application of molecular biology to plant physiology, plant genetics, and plant breeding.

Plant physiology, Biochemistry, and Basic molecular biology

General 1. Alberts B, Bray D, Johnson A, Lewis J, Raff M, Roberts K & Walter P (1998) Essential Cell Biology - An introduction to the molecular biology of the cell. Garland Publishing Inc. New York & London. 2. Baxevanis AD & Ouellette BFF (2001) Bioinformatics – A Practical Guide to the Analysis of Genes and Proteins, 2nd ed. John Wiley & Sons, Inc. 470pp. 3. Buchanan BB, Gruissem W & Jones RL (2000) Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists, Rockville, Maryland. 1367pp. 4. Cooper GM & Hausman RE (2004) The Cell: A Molecular Approach. 3rd ed. ASM Press, Washington, DC, USA.713pp. 5. Dennis DT & Turpin DH (1990) Plant Physiology, Biochemistry and Molecular Biology. Longman Scientific & Technical. 529pp. 6. Gibson G & Muse SV (2002) A Primer of Genome Science. Sinauer Associates Inc. 347pp. 7. Grierson D & Covey SN (1988) Plant Molecular Biology, 2nd ed. Chapman and Hall, New York. 233pp. 8. Glick BR & Pasternak JJ (2003) Molecular Biotechnology, 3rd ed. ASM Press, Washington DC. 760pp. 9. Hannon GJ (2003) RNAi: A Guide to Gene Silencing. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA. 435pp. 10. Hawes C & Satiat-Jeunemaitre B (2001) Plant Cell Biology, 2nd ed. Oxford University Press, New York. 338pp. 11. Heldt HW (1997) Plant Biochemistry and Molecular Biology. Oxford University Press, Oxford, New York & Tokyo. 522pp. 12. Howell SH (1998) Molecular Genetics of Plant Development. Cambridge University Press, Cambridge. 365pp. 13. Hughes MA (1996) Plant Molecular Genetics. Addison Wesley Longman Limited. Harlow. 236pp. 14. Hunt SP & Livesey FJ (2000) Functional Genomics. Oxford University Press, New York. 253pp. 15. Lea PJ & Leegood RC (1999) Plant Biochemistry and Molecular Biology. John Wiley & Sons, Inc., New York. USA. 364pp. 16. Lewin B (2004) Genes VIII. Pearson Prentice Hall, Pearson Education, Inc. Upper Saddle River, NJ, USA. 1027pp. 17. Saito K, Dixon RA & Willmitzer L (2006) Plant Metabolomics. Biotechnology in Agriculture and Forestry, Vol 57. Springer-Verlag Berlin Heidelberg. 347pp. 18. Thiellement H, Zivy M, Damerval C & Mechin V (2007) Plant Proteomics: Methods and Protocols. Methods in Molecular Biology, Vol 335. Humana Press Inc, Totowa, NJ. 399pp. 19. Vo-Dinh T (2005) Protein Nanotechnology, Protocols, Instrumentation, and Applications. Methods in Molecular Biology, Vol 300. Humana Press Inc, Totowa, NJ. 463pp. 20. Westhoff P (1998) Molecular Plant Development. Oxford University Press, Oxford, New York & Tokyo. 272pp. 21. Wu W, Welsh MJ, Kaufman PB & Zhang HH (2004) Gene Biotechnology. CRC Press, Boca Raton, Florida. 523pp. Lab manual 1. Draper J, Scott R, Armitage P & Walden R (1988) Plant Genetic Transformation and Gene Expression - A Laboratory Manual. Blackwell Scientific Publications, Oxford, London, Edinburgh, Boston, Palo Alto & Melbourne. 2. Gelvin SB & Schilperoort RA (1994) Plant Molecular Biology Manual, 2nd ed. Kluwer Academic Publishers, Dordrecht, Boston, London. 3. Gilmartin PM & Bowler C (2002) Molecular Plant Biology Volume One – A Practical Approach. Oxford University Press. 274pp. 4. Gilmartin PM & Bowler C (2002) Molecular Plant Biology Volume two – A Practical Approach. Oxford University Press. 332pp. 5. Micklos DA & Freyer GA (2003) DNA Science: A First Course. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA. 575pp. 6. Sambrook J, Russell DW & Sambrook J (2001) Molecular Cloning: A Laboratory Manual, 3rd ed. Vols 1, 2 & 3. Cold Spring Harbor Laboratory Press, New York. 7. Schuler MA & Zielinski RE (1989) Methods in Plant Molecular Biology. Academic Press Inc, San Diego. 8. Shaw CH (1988) Plant Molecular Biology - A Practical Approach. IRL Press, Oxford, Washington DC. 9. Tagu D & Moussard C (2006) Techniques for Molecular Biology. Science Publishers, Enfield, NH, USA. 227pp. 10. Weigel D & Glazebrook J (2002) Arabidopsis. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 354pp. 11. Weissbach A & Weissbach H (1988) Methods for Plant Molecular Biology. Academic Press Inc, San Diego. 12. Westermeier R & Naven T (2002) Proteomics in Practice – A Laboratory Manual of Proteome Analysis. Wiley-VCH Verlag-GmbH, Weinheim. 316pp. 13. Wilson K & Walker J (2005) Principles and Techniques of Biochemistry and Molecular Biology. Cambridge University Press, New York. 783pp.